“Things I wish I’d known from Day 1 of my PhD”

Collected by Ya’el Courtney, Twitter @ScienceYael, 2021

Prompt tweet: “Alright science twitter, I need your advice. I just joined a lab and started my  thesis work this week! Yay! What is the best advice you have for a new thesis student? Or, what  do you wish you knew or had started doing from day 1 of your PhD?”  

1. If you can get a handle on the ideas and theory behind what you are doing from the  beginning, you will be able to make better decisions as you go along! Reading textbooks  and articles – particularly from your own group – is a priority. @DrLEGs1 

2. Start writing your thesis early and add references as you go using an automated system  like OneNote. Treat yourself often, it’s an exciting but tough journey. *Expect* many  scientific failures, and dance with the breakthroughs. @DarylRhysJones 

3. Don’t attach your laptop to the back of a rickety bicycle…By which I mean have every  single document you write automatically update itself to cloud storage at very regular  intervals. @RoyleJoanna 

4. Congrats on starting your PhD! I used the first 3 months of my PhD for an intensive  literature review. I was able to write a basic introduction to my thesis (which came in  very handy 3 years later when I was so tired of writing!) and also knew lab methods I  wanted to test. @FiebigAntje 

5. Read this book – How to Take Smart Notes: One Simple Technique to Boost Writing, Learning, and Thinking – for students, academics, and nonfiction book writers. And learn  how to use Notion, a truly awesome app to create a 2nd brain. @donpollitt 

6. A PhD/thesis diary really helped me. I liked to do it first thing, free writing about the day  before. It also helped me to write a to-do list for the day ahead. I was able to see  connections I might not have seen, because I could reflect on my research in my own  way. @Alices_Films 

7. Properly define the question you are trying to answer. But don’t hesitate to revisit (consistently) this question in the light of new work, time, etc. Defining a proper goal is  key IMO. Make a lot of draft skeleton manuscripts (just the sections) in order to answer  it. @AounYouva 

8. Talk to your committee often and enthusiastically if you can. Try to win them over when  the stakes are low so your defense in a few years doesn’t feel so starkly different from  what you’ve been doing all along. @AndrewScarpelli 

9. Don’t fret the weeks when motivation lacking on a Tuesday afternoon shows up on a  Friday at 4 pm, just roll with it! When motivation for big tasks is missing in the moment,  turn to the small to-do items. @AnneMHartwell 

10. Find a citation manager that works for you and use it consistently. For me, @paperpile  has made organizing class readings, thesis readings, and comps prep SO much easier.  @Alissanneal 

11. First of all, congrats! Decide on a way to organize your computer files that makes sense  to you. Ask for feedback. It can be tough going from receiving grades semi-regularly to  having to be your own compass in assessing whether you’re on the right track. Enjoy the  ride ☺ @GuranCNA

12. Something my mentor told me 30 years ago that I share with students and new faculty  when they become overwhelmed – papers and grants are the coin of the realm & follow  your bliss. Good luck! @ZmudaJoseph 

13. I was my supervisor’s first student. My thesis failed – no PhD… make sure you gain and  practice real skills – mine have served me well for 30 years. @qqv0310 

14. 1. To avoid tears, back up your work (at least weekly). 2. Present your research as many  times as possible (to friends, colleagues, or lab mates). They will likely ask questions that  will help you. 3. Don’t compare yourself with others. 4. Don’t kill yourself (Enjoy your  PhD) @olayinka_saint 

15. Take your time – it’s a marathon not a race. Listen and hoover up hand-outs and papers  you are told to read. Start doing literature searches on your project and writing your  introduction as part of your reading around/learning your field. Write your thesis as you  go. @BarbaraGuinn1 

16. Read a paper every day and sort out a reference manager now @Montydoggolab 17. Hey, congrats! You can write all your notes about any text in Mendeley, if you don’t use  the info at the moment it can be useful later. @osirisglzgalvan 

18. Always remember, it gets hard before it gets easy. Surround yourself with people who  can support through the lows and celebrate your highs! @Kramayp 

19. Impostor syndrome will happen. But you still need to keep your own space without  guilt: go to the gym or do something you enjoy several times a week, leave work early  specifically for this. No work at home, you’ll be more productive when you’re there ☺ @_EvaMichaud 

20. Focus your own research topics and no need to compare your own achievements with  your peers. Different research topics take various amounts of time to be studied and  published. @DPsychai 

21. Read as widely as possible around the subject, see what’s already been done research wise in your topic area. @ValMansell  

22. Read lots of reviews and question everything. If protocol X says to use reagent A on  substrate B. Why that reagent? What does it do? Why that long? Why that  temperature? It is difficult to generate your own ideas if you just blindly do what everyone else tells you to do. @DerekFFleming 

23. Don’t pick a project right away. Instead, can you help out some postdoc or senior PhD  students with their work for a couple of months? It helps you find what appeals to you  and what is actually feasible to accomplish. @JerUpham 

24. Learn to dissociate failures of experiments from failures of yourself. A failed experiment  does not mean YOU failed! You just did science! @MariRSosa 

25. “I’ll remember everything is the biggest lie you can tell yourself” So write every small  detail down in your lab book or elsewhere. @NiranjanRamakr4 

26. Take notes. Also, citation manager is your best friend. @WojciechlTB 27. Analyse your results as you go and don’t write off anything until you have the full  picture! @Dannylasal 

28. Don’t forget that your PhD isn’t just 3 years of being a lab rat. Pay attention to the many  other skills you’re learning along the way. @EdwardRandviir 

29. Read every paper in your field – even the weaker-seeming ones @nitz_douglas

30. Besides the amazing advice from everyone, I would suggest please know that the best  networking in academia is with your current peers, not just with already established  profs, etc. I hope you will make great friends, because the PhD is made better with a  supportive community. @trang_mai_tran 

31. Learn grant writing as hard as you are learning research writing @ShalleePage 32. Read…take breaks, and the guilt will always be there. ☺ All the best. @Saydia_Razak 33. First, big congrats from a complete stranger! Second – it might feel like you’re getting  

nowhere in the beginning and that is completely okay. When I realized that, so much  self-induced pressure lifted and here I am 2+ years later wondering when I had time to  accomplish all I did! But as many others have said – treat it as an education and learning  process and make use of the immense knowledge you’re surrounded with.  @JuliaHoglundBio 

34. Take moments to enjoy the journey. @JLFetterman 

35. Find a productive, smart, non-angry mentor. @n3sgd1959 

36. Focus on what’s doable for your thesis. @EntoMike 

37. My advice: don’t read what you write until you finish the whole chapter. Rereading  sentences breaks writing flows. It’s easier to edit the content once you have it written.  And, there’s no such thing as a perfect thesis so don’t go through too many drafts. Link  to blog post “How I wrote a PhD Thesis and had the time of my life” 

https://www.talkplant.com/i-wrote-phd-thesis-time-life/ @TalkPlant 

38. Remember real science involves failure. Super tough to experience. But this is what  progress entails. When things don’t work remember: It’s not you and it happens to  everyone. & Learn to love your lab book! You won’t remember the next  

day/week/month exactly what you did! (&forgive yourself) The prime gain from doing a  PhD is nothing to do with the subject. It is life skills: surviving countless struggles,  persisting through difficult times, becoming resourceful problem solver & independent  thinker, and knowing when to ask for help. Invaluable for any future role!! @julie4clahrc 

a. Couldn’t have said it any better for me. Spot on. Surviving countless struggles  and problem solving. Not forgetting patience and persistence. @luciamamorena 39. Organize things systematically from the beginning. If you’ll be using  samples/strains/primers/plasmids etc assume you’ll have 100 or more by the end of  your PhD. Organizing things systematically now will help you find things later. In a few  years, you will thank your past self! @Linder_surprise 

40. WRITE IT DOWN! Everything, thoughts, protocols, papers, advice, sunny days, bad days;  be a good information giver to future you. @shellie_wall 

41. Make a git repository for your codes and log files (and write-ups if you are doing them  with Latex or markdown) per project. It helps you track them and have a back-up and  more importantly, you can revert if something went wrong. @neuroprinciples 

a. Hey I’m the author of @GitJournal_io – a mobile first markdown editor  integrated with Git. Maybe it will be useful for you to edit/view your notes on  the go. @visheshhanda 

42. Tons of good advice, perspectives, and fellow-feeling here. Helped keep me sane:  https://thesiswhisperer.com/ @DrRoseJones 

43. Know your strength and be confident @SambitBhoi3

44. Your supervisor is supposed to be a source of knowledge, motivation, and help! Go to  them with issues, you don’t have to struggle through everything alone. Also, start  reading and writing an annotated bibliography. It’ll help when you need to start writing  your literature review! @Andi1609 

45. Enjoy the ride. @alexhdezgcia 

46. Find allies. @Teresa_GB 

47. Experiments fail 90% of the time in your first year and 35% of the time towards the end.  @AnanyaS1190 

48. I’ve never been able to find it again, but I once saw a tweet that just said ‘learn to code  and go to therapy’ and I remember wishing I could tell that to my first year self.  @gottapatchemall 

a. Oh my God this is so true, not learning coding and going to therapy earlier were  also my regrets! @trang_mai_tran 

49. Have a consistent date/year/name system for all your files and create a master list with  a brief description of what the files contain! @ZJAyres 

a. Recommend something like ISO8601: YYYY-MM-DD hh:mm eg 2020-07-09 15:45.  Easy to read and understand, international standard means it’s easier for collab  and for computing. Sorting alphabetically = sorting chronologically.  

En.wikipedia.org/wiki/ISO_8601 @GentScientist 

i. Would avoid using spaces in file names. I’ve had issues with spaces when  using such files in coding/programming. But the date/time format is  

indeed great, consistency is key. @NinjaaNina 

1. Very fair. Depending on the programming language (eg bash  

scripting) can be an issue. Proper ISO8601 puts a “T” in that space  

for pretty much this reason, I expect. @GentScientist 

50. Write three related papers, publish each, and make each a chapter in your five chapter  thesis. @DrAdamWarren 

51. So 1) CONGRATULATIONS learn to celebrate the victories and 2) take the time off. All of  it. The weekends (yes the whole two days), don’t do 12H in the lab every day, and take  holidays. Yes, WHOLE WEEKS OFF. I’ll die on that hill. It’s a marathon, pace yourself or  it’ll be painful. @lu_debeauchamp 

52. My data organization was so fucked up at the start! My best advice to you si to get  everything down in clean spreadsheets, and label adequately so you can refer back to it  in a few years. What I didn’t know at the start was that I’d wind up doing almost all  behavior experiments, and then after that really fall deep into data science kinds of  work. I don’t think I could have planned that from the start but perhaps that means it’s  best to keep an open mind. Like if your first, or best, or favorite plan doesn’t pan out,  that’s a reflection of the state of things rather than a reflection of you. We can influence  a lot! But not completely, so try to unburden yourself from taking absolute  responsibility to “make” things work. We can still do “good” for a “bad” project, and you  still take the credit for good work with you. I hope you take encouragement to heart  from all this! And a realistic notion that challenges will present themselves. But you are  more than up to the task! @keireton

53. Collaborate if you can & going to meetings helps with this. It’s how I obtained the  human microglia cell line which mas meant everything to my research. I try to also make  at least one new friend at each conference. @GliaAlzheimers 

54. Don’t ever procrastinate genotyping, and second having a good organizational system to  keep track of everything! (especially if you generate a lot of tissue for histology)  @andreayung3 

a. Yes! Organization! When given freezer space, number all of your boxes and keep  a spreadsheet where you list what is in each box. Keep this spreadsheet  

updated! Digging around the -80 for samples from 4 years ago is no fun.  

@krissylyon 

55. Set up a thoughtful system/format for recording what you’re doing every day (physical  lab notebook, digital notes, etc) and for labelling/storing files and samples NOW instead  of later. Don’t be afraid to ask for help, and expect to mess up a bunch ☺ @hcmacomber 

a. Couldn’t agree with the labelling/storing of files more!! @SophieFarrow2 56. Document everything. Literally everything. I’ve had good luck by keeping an overleaf  document open all the time. Every day, I review the previous day and make a brief to-do  list. Useful notes, paper summaries, computations, end-of-day report, etc. get added  before I go home. When it comes time to write your dissertation, you may well be able  to form much of it just by copy-pasting from your notes. @koaleszenz 

57. Agree with others on organization and note-taking. But also don’t be too hard on  yourself. There will often be someone better than you at a particular thing, but rarely  anyone better than you at everything! @asa_barth_maron 

58. Keep records of everything that is discussed with your mentor in meetings. Some  mentors can be nightmares (hopefully not in your case!) and it’s a good idea to have a  paper trail. Others will be forgetful of what they told you to do (they have a lot going  on!) @leannakal 

59. It’s a Phd. It’s not a PhA+ @HlthyUncert 

60. Woohoo, congrats! I wish I knew how to pace myself/find balance. I spent too much  time worrying about exceeding everyone’s expectations that I forgot to take care of  myself along the way. No experiment, grant, or manuscript is more important than  taking care of you! @BrittanyUhlorn 

61. This video is really helpful! I wish I had watched it at the start of my thesis work. “Don’t  perish! A step by step guide to writing a scientific paper”  

https://www.youtube.com/watch?v=LUmf7vEFxYI&feature=youtu.be @FungalEvoDevo 62. Story board your project early on. It will help you keep track of the thread of your  research, identify the best future directions, and easily transitions to an outline for a  paper. @krissylyon 

63. Take any training offered. Need more? Ask for it. Backup everything. If you have  problems tell your supervisor sooner not later. PhDs take longer than you think.  Research plans always change. Don’t read the “how to get your PhD” type of books the  week before your viva. Enjoy! @DrPetra 

a. I would like to second all of this, but especially “backup everything”. My  computer started to die on me in the last year of my PhD. @jesskorte

b. These are so true. I wish I had known them too. @trang_mai_tran c. This is a GREAT response. Embrace what you’re there to do and enjoy it.  @DavidBarker_PhD 

64. Write yourself little cheat-sheets for every new process or programming tool you learn.  3rd year you will thank yourself for not having to re-learn how to rotate those figure  labels in ArcMap at 3 am the day before an abstract deadline. Look up bullet journaling  – helped me keep my thoughts, meetings, notes, hopes, dreams, phone numbers,  sample names, to-do lists, podcast recommendations together without needing 200  different notebooks. @AnnaGlueder 

65. Pick a project your lab has a lot of expertise in. Ideally, work closely with a senior grad  student or postdoc for a year or two, get some second authorships, then forge your own  path. @jbohnslav 

66. Read, read, read @DanLevy919 

a. I just told that for the Nth time to my trainees today: read, read, read. Papers are  like books, every time you read one, there is another layer popping up.  

@AdemaRibic 

67. Write every single thing down in a single location like a lab notebook! You may think,  there’s no way I’ll ever forget that. But then a pandemic may hit and remove you from  lab work for 3 months making you forget something you once thought was so trivial.  @slspeckhart 

68. This excellent article by @LucyATaylor is a goldmine: Twenty things I wish I’d known  when I started my PhD https://www.nature.com/articles/d41586-018-07332-x @Prokaryota 

69. 1. EVERYTHING takes longer than you think. Don’t rush. Focus on the quality of your  work rather than at the pace at which other people are working. 2. Don’t be afraid to  talk to your colleagues about your struggles. The chances are that they’ve struggled with  a similar thing. @EmilySpearing 

70. Sit down and really hash out expections with your mentor, theirs & your own, for  experiments and for other lab work, writing, professionalism, you mentoring others, etc.  Make a timeline for project goals, milestones (exams/proposals, grants), career  advancement. READ THEIR GRANTS! @HowardNeuroLab 

71. Schedule weekly 1-on-1 meetings. Solicit feedback every chance you get. After your 1- on-1 meetings, send your PI a short written synopsis (bullet points) of what you  discussed) @BrumbackLab 

72. Back up all important files in two places including one external drive. Document  everything and number your notebooks. Don’t panic if something doesn’t go to plan the  first time. Enjoy it! @PixAmes 

73. Eventually, everything is about perseverance. There will be times you’ll think – why am I  doing this? What if I was more suited for something else? There will always be someone,  something better out there. The only thing that will bring you joy is commitment no  matter what. This reminds me of relationships. There will always be someone more  loving, pretty, and accomplished in the world who would have been an objectively  better match. But you don’t need what is the best. You just need something that works  for you. Questioning what you’re doing is not unhealthy. Rather, it is the sign of an 

objective mind, that cares for its own well-being and future rather than putting  everything on a pedestal and worshipping it. It can give you perspective, and accepting  your doubts makes you enlightened. @Anirudh_Ramesh1 

74. Congrats! I agree with @BrittanyUhlorn – I’d have loved to pace myself and find  balance. It’s so easy to get caught up in competition in grad school. Remember,  everyone’s on their own journey. Make generous interpretations of their (and your)  actions. Be gentle with yourself. @RaghavSampangi 

a. Exactly – run your own race! So much easier said than done when success in  academia is often based on our numerical competition with others (pubs, grants,  etc) but stay true to yourself! @BrittanyUhlorn 

75. Never trust yourself to remember a small detail that may someday be important. Write it down! Preferably somewhere you know where to look. @RosemaryChicken 76. Last year I was on the panel for a workshop at our university called ’10 tips for new PhD  students’. Afterwards I wrote my tip in this article: “Your PhD is a journey – enjoy the  ride’ https://www.linkedin.com/pulse/your-phd-journey-enjoy-ride-evelyne-deplazes/ @DepalzesEvlyne 

77. No matter how well intentioned and prepared you are, eventually your PhD will feel like  walking in the forest and your flashlight goes out. Give yourself some time to adjust  rather than beating yourself up for being unprepared. @AnnaGlueder 

78. I don’t need ‘fastest/best/most consistent email responder’ to be part of my  professional legacy. I may be a student, but still I am human. Other people’s timelines  aren’t always my timelines and rarely is anything truly an “academic emergency”.  @sjcherak 

79. I’m at the end of mine…take notes on literally everything you do. This will be invaluable  when you are writing your thesis. Also, check the replies here:  

https://twitter.com/RobinMazumder/status/953397393604665344 (around 1000 replies,  highly recommend checking this out as well!) @RobinMazumder 

80. Write in a book absolutely everything you are doing! Every reference product, every  product lot. It will help you avoid some mistakes and save time when you have to put all  the data together for a paper. You can’t remember everything after some years of work  ☺ @AurelieDobric 

81. It always takes longer than you think. Document well everything. Get used to planning  all of your experiments from start to finish. Don’t wait with analysis too long. It’s just  work, don’t let it take over your life. Reward yourself. @RekaPToth 

82. If something is going to take <5 minutes, do it now. Takes way less effort than having to  remember to do it later. Label every sample like they’re someone else’s, because future  you is someone else. It’s okay not to know things. @KateKennedy 

a. Excellent advise from Kate! Also come up with a system for labeling computer  files and stick to your system – will make it easier to find things later. Never too  early to start writing your thesis – make your figures and write your methods as  you go. @Steeltown_Worms 

83. Learn about Mendeley. Stay consistent with labeling and note keeping practices. Back up your work regularly. Ask all the questions needed until you understand the purpose 

of your experiments. Be honest about your struggles. Find a community/friends.  Breathe and have fun. @CaroMaldonado1 

84. Please check out all these posts by the wonderful @MollieMarr! She is a huge  inspiration. The thread also contains a ton of wonderful advice.  

https://twitter.com/MollieMarr/status/1280711793413742593 @BrittGratreak 85. Make sure to do something you love outside of the lab. I played in a wind ensemble throughout my PhD program. It wasn’t always easy, but it was very important for self preservation. @eyedoc333 

86. Trust yourself, have faith, you don’t need any advice. I was all worried and anxious when  I started my PhD and at the end of the years realized to trust the journey.  @Anonymo74045043 

87. Figure out a good system for keeping track of what you’ve read and what the main  takeaways were. Reference managers are good, but may not be appropriate for this  task. @DJWeduwen 

a. I think roam research or obsidian are great. Also notion is awesome for projects  management. @esl3m7anafy 

88. Learn a reference management system. I use latex and bibtex. Keep everything you  need for your thesis – notes on your process, papers you think would be helpful. Keep a  note of all code you generate for statistics. I can’t tell you how many times that last one  has saved me. @cooper__hodges 

89. Planning and creating figures of experiments even if I think they don’t work out. Saves  so much time later. Start writing from Day 1. Enjoy it ☺ @JessicaL_Davis 

90. Don’t get obsessed with learning every technique that seems useful. Good rule of  thumb is if you’re going to do it more than 6 times, learn it. Otherwise collaborate. Do  find a couple specialty techniques to master. Do every experiment like you’re going to  put it in a paper. More neurospecific: Learn matlab (imaging) or Python (omics) right  now. learn Opti-patch or similar setup if interested in neurophys. Collaborate with the Greenberg lab if at all possible. @zenobiotic 

91. Congrats! Document, document, document. Get a citation manager and put everything  in. Record everything you do in lab and make backups. All the backups. Eat 3 balanced  meals a day. Get sleep. Get exercise. Don’t give those things up for more lab time, it’s a  false economy. Don’t neglect your relationships and take time to build new ones. You  need supportive people in your life – they’re the ones who will cheer you on and help  you through when you feel like you will never finish. If you’re struggling, don’t wait to  ask for help, do it asap. @ashmeads2 

92. Read the relevant literature. You probably have decades of research to become familiar  with. Network. Do internships. Check in with your advisor often, write in your lab  notebook like it’s your diary, backup your files often, and use consistent formatting  across your files. @okieokra 

93. Read the papers published from the lab you are in going back ~5 years. @gtrubl 94. Just let’s see by yourself 2-3 month. Take time to observe the (wild life) lab. Don’t be shy  to ask from postdocs anything. Then you can start and try all the good advices from  people if you want but a PhD is kind of a unique experience imho. @AnthonyGuihur

95. That I could have had undergraduate or master’s students helping me in lab and it  would have been mutually beneficial. Know your resource pool! @DrCaplin 96. Keep good notes from the start because it’ll make the writing phase so much easier  @hsanderson_phd 

97. Use Git for version control of your writings (in LaTeX or Markdown) and enable  spellcheck. @fredfeng 

98. Find a good therapist and set a clear plan and objectives with your supervisor. Don’t be  too hard on yourself, you will do great! @ZeinabBakhtiar1 

99. @anniegorden 

100. Just observe the lab culture for a while. Then slowly adapt to the circumstances and  experiment, experiment like the wind! Good luck! @Baldscientist 

a. I really like this advice. Lab culture is so very important and deeply affects how  each individual will conduct their research. @vinisalazar_ 

101. Find a way to keep track of what you read – on paper or digital, write out the main ideas,  data sets, methods so you can quickly find “that one paper that said X” when you go to  write w/out having to rea-read everything (and it’s already in your own words!)  @April__Abbott 

a. If you go the handwritten route, just remember than there is no ‘ctrl+f’ even if  you “swear you have written this factoid that explains your entire dataset  somewhere in these 60 pages” @AnnaGlueder 

102. Organise a meeting schedule with your supervisors from the start and stick to it, even if  some weeks/fortnights you have nothing much to report! @tansybranscombe 103. Treat your PhD as a training, not a degree you need to get to go on to the next stage of  

your career. In hindsight, I wish I wasn’t so set on the end goal and soaked up all the  possible skills I could. @DrJMatharu

Be kind to yourself. It’ll be so easy to compare yourself to your advisor, labmates, cohorts, and  friends. But don’t worry about what they’re doing, worry about what *you’re* doing. You’ll  make tons of mistakes, but you’ll be a better scientist for it! Just do the best you can!  @neobhm 

104. Make super quick “figures” of every experiment you can. No need to get fancy (paste 1-2  images/results in a ppt slide, brief annotation), but this will really help when you go to  prep grants, papers, committee meetings, etc. Also, try to establish a solid relationship  w/ your PI, including regular meetings. You’ll want that foundation to hold you up during rough patches (scientific and personal). Like others have said: establish your file  structure, and make annotating and backing up your data a habit. @KtTots 

105. What I wish I knew, and what is still true, is that established scientists are not Gods (not  even small g gods!) – challenge, push, poke, prod, follow your curiosity.  

@JamesDanckert 

106. Most important advice: don’t give up. There will be tough times and that’s the nature of  research, but you’ll prosper. If you feel down, take a break and relax for a bit, then get  back at the challenge. @farahikia_m 

107. Focus on your thesis work and don’t get carried away with side projects @tamtav1 108. Start using a citation manager on day one @vbarber830 

109. Get on a schedule that works for you. Take breaks. Buy an external hard drive. Advocate  for yourself as needed!!!! @jesstcmpbll 

110. Imagine looking back on your data from five years ago – does it make sense to you now?  Do you have 15 versions of the same file and they’re all slightly different and you don’t  know why? Get organized from the beginning! @KVanVolkom 

111. Read as many papers as you can, but don’t sit in an office all day. Get in the lab and  talking with people. My best ideas came early in my PhD when I had the opportunity to  pursue whatever I wanted to! Most of all, enjoy!! And be proud of yourself <3  @savvy_science 

112. Don’t compare yourself to other people! Everyone’s research and rate of publication is  different. @brooke_kuei 

113. Plan ALL your projects at once and have your committee agree in writing on all project  protocols. No playing games this way. Limit any dependencies in designing said  protocols, such that they can all be carried out together and there is no waiting. Be  scrappy, just do it. @kannamma1234567 

114. Have a physical lab notebook and write down everything (protocols, experiments, the  date of the experiments) Learn the techniques, and ask for help. Read two articles a 

week. Write everyday at least one sentence. Powerpoint for the data and keep it  updated! @dr_dania87 

115. Do original research that you care about @jackcs99 

116. Record incidents of harassment that happen: date, time, witnesses. Don’t allow others to  make your workspace unsafe/uncomfortable. Labmates are coworkers, not your friends.  Get a good citation manager (Mendeley/Endnote/Zotero). Try to join poster/oral  competitions even if you don’t think you have enough results to win. Set weekly or  biweekly or monthly meetings with your PI. Collaborate with others as much as you can.  Do a term abroad. Your value does NOT equal your progress/success. Period.  @jerichobreeze 

117. I Wish I knew about the #Notion #app. Use it to document your literature reviews, collect  meeting notes, all in one place. The best part about this app is that it works on windows,  MacOS, android, and iOS. Whether you are in the shower or in lab, note down your  ideas quickly using the fantastic templates that the app offers. @KarthikPN15 

118. Now’s the time to set up good habits! Reference management, any new programming  languages or software you want to learn – look at what is best right now and go for it.  It’s a good investment. Also start writing as much as soon as possible. You can refine  later but any papers you read (add some review notes/to a lit review) add even small  experiments to methods, add random bits of interpretation to a results/discussion  section. All helps. Do other things to supplement your skills for post-PhD employment  prospects – attend broad seminars, collaborate on tangential projects, communications  work, contribute to local policy papers, organize events. ☺ @HayleyEversKing 

119. The thesis work you started this week is not the thesis work you will be graduating with,  so don’t get too attached. @SugiuraAyaka 

a. So true, especially when you are about to read your supervisors’ comments on  your draft. @trang_mai_tran 

120. Make a note of every tiny bit of information at every step of the way, even things that  seem completely irrelevant. You’ll definitely thank yourself later when it’s time to  compile everything and write that thesis up. @susanj13 

121. Have a labeling system for your projects and experiments! I’m doing it now in my last  year and I wish I had started from day 1. @AbbeLimitless 

122. Make time for reading! Might just be me but I got very focused on lab time at the start so  dedicating X hours for reading was helpful to keep up with what’s going on. Good luck  and enjoy! @Perry__SC 

123. Now is the time to start your post PhD planning and positioning: Postdoc -> faculty ->  industry, etc. Your plans will likely change but that’s okay. Also invest time in curating a  broad library of references with a citation manager. (Jabref, Papers, etc) 

@PhysDownUnder 

124. Realize that everything in a PhD takes a lot longer than timelines you were used to as a  single researcher. Patience! Have a document to save discussion points and topics into  from the beginning when mentioned by supervisors in discussion/drafts… good luck!  And enjoy ☺ @WoodwicksLianne

125. Yeah…back up everything…always. Keep raw data and analyzed data together, same file.  Lastly, think about the controls/experimental design before you run an experiment; it’ll  save you tons of time. Allow yourself to make mistakes. @stern_sydney 

126. ORGANIZE YOUR FILES. Make lots of subfolders and name files with dates instead of  “version”. Organize them as if your advisor is going to one day open up your computer  to find something. Your future self will be searching for that one file, and you will thank  yourself. @Amandisols 

127. 1. Save everything on the cloud. 2. Start your lit review as soon as possible. 3. Get in  contact with other researchers @cheagarcia 

128. Enjoy the ride. Work hard for yourself and not to impress someone else. Most  importantly, always remember that your work is just as important as your neighbor’s no  matter what happens. You got this! @elizabethchau 

129. Accept that you will only ever do 1/10 of the research in which you are interested during  your thesis. You will have to say no to things you are really interested in so you can stay  focused. Acknowledge and let these projects go. You can come back once you are a  doctor. @MAllyse 

130. You may end up throwing away everything you do in the first year. This is OK – the first  year is (or can be) for exploration. I completely pivoted at the end of my first year, and  my thesis ended up being about something quite different. @jimfinnis 

131. Ask all the questions!! You’re here to learn and senior students were first years too.  @Roxane_jourdain 

132. Spend some actual work time to organize all your files in your PC thinking about that you  will need them in 3 years time. Then, read a lot of literature. That’s what I would say to  myself during day 1 of my PhD. @AkkindinS 

133. I wish that I was more diligent about recording everything in my notebook while I’m  running my experiment, even if it’s a generic process I’ve done many times. If makes  writing and thinking about my results much easier. @ABAChemE 

134. Perform every single experiment in a way that it can be used for publication. Don’t ‘just  briefly check quick and dirty’… @genderwoche 

135. ORGANIZE YOUR DATA AND LABEL EVERYTHING. That is all. My old mentor from my PhD  program said today “Don’t judge your self worth from your CV.” And when you create  any figure, for the love of everything, write down what you did, parameters, work flows.  SO VITAL. You will thank yourself later. You remake same figure hundreds of times.  @SassySeaShellss 

136. Complete all data entry/photo uploads as quickly as possible, have a dedicated notebook for everything project related (I have one for notes from all meetings and one for all  project notes), and give yourself a lot of grace! @hortmeierclarke 

137. Start a running annotated bibliography of everything you read! @GenevieveGoebel 138. Figure out a way to name your files and folders that makes sense and use it!  @AmyKeirstead 

139. Write write write as you go don’t don’t don’t wait until the end to write it all up  @ShariHilding

140. Keep two “lab notebooks” so you can take one (protocols good reagents etc) when you  leave cause you *will* forget that ‘one random german company that made the BEST  antibody’ @kharris1287 

141. Analyze your data as soon as you get them! @Mehrnaz_Mjt 

142. 1. Always create at least 2 backup files for data 2. Embrace flexibility 3. The research you  end up doing is going to be nothing like the one you had conceived of in your SOPs 4.  Maintain an excellent relationship with your guide. @JayantikaChakr1 

143. 1. Vent to and with your peers to see everyone struggles sometimes. You’ll learn about  resources and you’ll be okay. 2. Backup everything. Then backup again. I lost my original  and first backup of all my data at once in my final year. Second backup = why I’m Dr.  Nevins. Remember: you got this. @JuliElyHa 

144. 1. Learn how to read a paper (the three pass technique) 2. Become a peer reviewer early  enough to understand the other side 3. Attend as many seminars in allied areas as  possible 4. Do few internships 5. Mentor UG/PG students when you are ready  @YusufGupta 

145. Loving myself & everyone around me @DrGuilllotWright 

146. Read as many papers as possible, try for at least one a week! Write everything down so it  makes writing your thesis wayyyy easier. @chelsdoingsci 

147. Be aggressive about communicating frequently with your mentor. Schedule frequent  meetings with your advisory committee as well so that you can stay on track. It may feel  intimidating, but ultimately their job is to help you graduate, so use this resource. Be  your own advocate. Also, ask other labs, students, faculty for help with new techniques. @howdy_comrade 

148. Maintain an impeccable lab notebook. @Fahdqadir 

149. Treat this not only as job, but a hobby! The weirdest but best advice I ever got was – does this keep you up at night? If it does, you will do very well! @DVogtLab 150. Believe in yourself. You’re going to doubt yourself. But turn that around and do what you  need to pump yourself up! And most importantly write everything you do down – record it! @science_SRH 

151. My thoughts 1) pick the right project (exciting, cutting edge, tangible) 2) learn all you can  about your lab members and their work (talk, talk, talk) 3) start doing broad  experiments to learn more about the lab @Plasmacellguy 

152. Find the senior postdocs and staff scientists that have done your techniques and types of  research for more years than anyone likes to admit. Get their gold standard protocols  and use them exactly as written… that’ll cut at least 6 months off of your PhD.  @anthony_berndt 

153. The best advice I received was “write down exactly why you make every decision”. In the moment you will think it is obvious and you will definitely remember. But months/years  later it isn’t and you won’t. So my advice: detailed notes. Write down as much as you  possibly can. @courtbouch 

154. Question everything, do your controls early, and push back if you think your PI is asking  too much. Also, have fun, research rocks @Scalebar 

155. Have fun and never give up @anaslaaa

156. Take organized notes on all the papers you read! Will come in super handy when  preparing manuscripts and your thesis down the line (advice I was given) @jamietcard 157. Follow @EricTopol @nickisnpdx 

158. Manage literature in a way that will make it easy for you to cite it when writing  manuscripts. Here’s my favorite method by @SteMcQuilliam and I wish I had known it  sooner: https://twitter.com/SteMcQuilliam/status/1252241904810557448?s=20.  @Spring_Cyclone 

159. Definitely try to plan in advance professional development in areas other than technical  science. E.g. communication, managing up, developing a holistic portfolio of experiences  and learning that prepares for academia and industry And a mentor (not your  supervisors) @HossaiGul 

160. My suggestions are minor and more operational: pick a knowledge management  approach early, learn it and stick with it (incl ref management); have at least two  ongoing, separate, and non-deletion propagating backups; and think about writing  habits e.g. https://blogs.lse.ac.uk/lsereviewofbooks/2017/06/16/book-review-air-light time-space-how-successful-academics-write-by-helen-sword/ @ben_hr 

a. Yes! That book is a fav, and Helen is running online writing workshops atm. At  the start even simply writing your critical reflections of literature that you are  reading is a good way to learn and get into writing habits. @HossaiGul 

b. I hesitate to mention it, as it’s not for everyone, but thinking about a  zettelkasten forces one to consider knowledge management in general @ben_hr c. I didn’t know what a zettelkasten was until just now! But I’ve had an annotated  knowledge management system with a search function using evernote the whole  time. Plus systematic reviews kind of force you to get used to it! @HossaiGul d. Yeah I jumped ship from Evernote to @zettler last year ☺ @ben_hr 

e. Oooo a non-techie markdown editor! Might spend time on weekend checking it  out. Thank you Ben! @HossaiGul 

161. Unionize your program or risk having your funding/benefits cut during a pandemic.  @sydewolfe 

162. Best advice, let it go, this shit messes with your brain. @ratherhanan